Marian P. De Leon is a microbiologist with a PhD in Engineering (Molecular Biotechnology) from the Osaka University, Japan. She also holds MS and BS Degrees in Food Science and Technology, respectively from the University of the Philippines Los Baños.

From 1997-1999, Marian has attended specialized research fellowships in the International Center for Biotechnology (ICBiotech), Osaka, Japan with grants coming from the Asian Network on Microbial Research at Institute of Physical and Chemical Research.

She is a member of the Philippine Society for Microbiology, Inc.; National Research Council of the Philippines; Mycological Society of the Philippines, Inc; Japanese Biochemical Society; Protein Science Society of Japan; and the Gamma Sigma Delta – The Honor Society of Agriculture. Marian is also the current president of the Philippine Network for Microbial Culture Collection, Inc.

Marian has published and deposited three protein crystal structures and coordinates of Tk-subtilisin derivates in the Protein Data Bank.

Selected Publications

Monsalud, R.G., EP Castillo, GR Dedeles, MAP De Leon, MAT Siringan, LM Florento, EL Brillante, AR Creencia, NH Tan Gana, CB Brown, NSC Tabao, M Ritumalta, VB Argayosa and SSP Bulaong. 2012. Directory of Culture Collections in the Philippines. 3rd edition.

Pulido, M.A. 2008. Microbial Biotechnology. Institutionalizing Biotechnology as a General Education Course in Philippine State Universities and Colleges. A joint project of National Institute for Molecular Biology and Biotechnology, Commission on Higher Education and Department of Agriculture-PIA.

Pulido, M.A., Tanaka, S., Sringiew, C., You, D-J, Matsumura, H., Koga, Y., Takano, K. and Kanaya, S. 2007. Requirement for left-handed glycine residue for high stability of the Tk-subtilisin propeptide as revealed by mutational and crystallographic analyses. J. Mol. Biol., 374: 1359–1373.

Pulido, M.A., Koga, Y., Takano, K. and Kanaya, S. 2007. Directed evolution of Tk-subtilisin from a hyperthermophilic archaeon: identification of a single amino acid substitution responsible for low-temperature adaptation. Prot. Eng. Des. Sel. 20: 143-153.

Pulido, M.A., Saito, K., Tanaka, S., Koga, Y., Takano, K. and Kanaya, S. 2006. Ca2+-dependent maturation of Tk-subtilisin from hyperthermophilic archaeon: Propeptide is a potent inhibitor of the mature domain but is not required for its folding. Appl. Environ. Microbiol., 72, 4154-4162.

Pulido, M.A. and P.C. Sanchez. 2001. Catalogue of Microbial Strains. Museum of Natural History, UPLB. ISBN 1655-3845.